In addition, recommended screening strategies are presented where a sequential approach and Design of Experiments are used and discussed. The medium combines high rigidity with high dynamic binding capacity (DBC) and.
#Capto mmc impres binding capacity how to#
To use the full potential of Capto S, we show how to thoroughly screen the loading parameters, conductivity and pH. Capto S is a chromatography medium based on the novel high flow agarose platform. It is possible that the larger BsAb sterically hinders some binding sites and thus reduces overall binding capacity. it will be advantageous to identify the dynamic binding capacity (DBC) of the Protein. Capto S is a strong cation exchanger designed for capture and intermediate purification of recombinant proteins and as a second step in MAb purification. An example of such a column is a Capto MMC, Capto MMC ImpRes (GE. When using Capto MMC ImpRes, protein binding can be expected. Columns for screening of selectivity, binding, and elution conditions, as well as for small scale purifications. Previously launched products on this platform include Capto Q, Capto MMC and the MabSelect™ family. The capacity for selective removal of some key contaminants may decrease at high flow. The medium combines high rigidity with high dynamic binding capacity (DBC) and fast mass transfer to allow faster purification, more flexible process design and maximum cost efficiency. This has recently been used in the development of a new cation exchange medium, Capto™ S, and gives a substantial increase in binding capacity compared to the corresponding media without dextran.Ĭapto S is a chromatography medium based on the novel high flow agarose platform.
Removal of contaminants such as aggregates, host cells, and leached protein A in either bind- elute or flowthrough mode. A technique for significantly improving the capacity and mass transfer properties of ion exchange media is modification of the porous base matrix with a surface extender such as dextran. Polishing Capto MMC ImpRes is designed for post protein A polishing of monoclonal antibodies. Increasing expression levels of recombinant proteins and the need for improved productivity and overall process economy puts extra demand on the next generation of chromatography media for the bio-pharmaceutical industry.
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